Record Details

Estimation Activity And Partial Purification Of Leucine Amino Peptidase (Lap) In Patients Wiith Diabetic Nephropathy

Baghdad Science Journal


Field	Value

Title	Estimation Activity And Partial Purification Of Leucine Amino Peptidase (Lap) In Patients Wiith Diabetic Nephropathy

Creator	Journal, Baghdad Science

Description	Leucine aminopepotidase (LAP)[EC:3.4.11.1] activity has been assayed in (50) serum samples of patients with diabeties naphrophathy D.N (non-insulin dependent diabetic (NIDD) , and (50)serum sample of healthy individuals without any clinically detectable diseases have been as control group. The aim of this study is to measure leucine aminopeptidase activity and partially purifying the enzyme from sera of patients with diabetes nephropathy The results of this study revealed that Leucine aminopeptidase (LAP) activity of nephropathy patient’s serum shows a high signifiacant increase (p < 0.001) compared to that of the healthy subjects.LAP was purified from the serum of patients with diabetes nephropathy by dialysis and gel filtration (Sephadex G-25) (fine ) (20 × 1.5 cm ) .A (1.37) fold purification of serum LAP from patients serum with diabetic nephropathy was achieved by using dialysis and this enzyme showed single grade increased to (8.33) fold by using gel filtration Abbreviation: Leucine aminopeptidase=LAP, Diabetes Nephropathy= D.N, Non- Insulin dependent diabetic= NIDD.

Publisher	College of Science for Women - University of Baghdad

Date	2012-12-02

Type	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article

Format	application/pdf

Identifier	http://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/1414

Source	مجلة بغداد للعلوم; مجلد 9 عدد 4 (2012): issue 4; 689-694 Baghdad Science Journal; Vol 9 No 4 (2012): issue 4; 689-694 2411-7986 2078-8665

Language	eng

Relation	http://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/1414/1345

Rights	Copyright (c) 2012 Baghdad Science Journal